• INRS
  • Eau Terre Environnement
  • Énergie Matériaux Télécommunications
  • INRS – Institut Armand-Frappier
  • Urbanisation Culture Société
Version imprimable

Nicolas Doucet

Ingénierie enzymatique, RMN, dynamique

Dynamique moléculaire impliquée dans l’activité catalytique des enzymes


Research Interest

Understanding the importance of residue motion in enzyme activity may eventually allow us to develop methods to modify, modulate and/or design new protein functions, which would have far-reaching implications in biotechnology, protein engineering, nanotechnology and drug design.

The main goal of our research is to successfully modify enzyme biocatalysts aimed at environmental and pharmaceutical applications, with a broader interest focused on understanding the role between structure, function and flexibility in various enzymatic systems.

To address this important problem, our research strategy combines directed evolution experiments coupled with biochemical and biophysical characterization such as nuclear magnetic resonance (NMR), molecular biology, enzyme kinetics, and molecular modeling. Using this approach, we specifically focus on achieving the following aims:

  1. To understand how the amino acid sequence and local structural environment of conserved residues determine the motional and functional properties of enzymes.
  2. To investigate how functionally and structurally related enzymes retain their motional properties within similar folds.
  3. To modify, reproduce and apply this 'structure-function' and 'flexibility-function' information to the design of new and improved molecular biocatalysts.



Dr. Nicolas Doucet obtained his B.S. in biochemistry from Université Laval (B.S. Biochemistry, 2000) and successively completed a master's degree (M.S. Biochemistry, 2004) and a doctoral thesis in enzyme engineering at the Université de Montréal (Ph.D. Biochemistry, 2007). After completing a protein NMR postdoctoral fellowship in the Department of Chemistry at Yale University (with J. Patrick Loria, 2007-2010), Dr. Doucet joined the faculty at INRS-Institut Armand-Frappier in 2010.



Consultez notre webzine



Nouvelle approche thérapeutique pour le traitement du diabète de type 2 ...


Le professeur Nicolas Doucet parle de l’ingénierie des protéines ...


Le gouvernement du Canada investit dans la recherche universitaire de l’INRS

Le gouvernement du Canada investit dans la recherche universitaire de l’INRS